Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.23/576
Título: Design of cationic lipid nanoparticles for ocular delivery: Development, characterization and cytotoxicity
Autor: Fangueiro, JF
Andreani, T
Egea, MA
Garcia, ML
Souto, SB
Silva, AM
Souto, EB
Palavras-chave: Olho/metabolismo
Soluções Oftálmicas
Data: 2014
Editora: Elsevier
Citação: Int J Pharm. 2014;461(1-2):64-73
Resumo: In the present study we have developed lipid nanoparticle (LN) dispersions based on a multiple emulsion technique for encapsulation of hydrophilic drugs or/and proteins by a full factorial design. In order to increase ocular retention time and mucoadhesion by electrostatic attraction, a cationic lipid, namely cetyltrimethylammonium bromide (CTAB), was added in the lipid matrix of the optimal LN dispersion obtained from the factorial design. There are a limited number of studies reporting the ideal concentration of cationic agents in LN for drug delivery. This paper suggests that the choice of the concentration of a cationic agent is critical when formulating a safe and stable LN. CTAB was included in the lipid matrix of LN, testing four different concentrations (0.25%, 0.5%, 0.75%, or 1.0%wt) and how composition affects LN behavior regarding physical and chemical parameters, lipid crystallization and polymorphism, and stability of dispersion during storage. In order to develop a safe and compatible system for ocular delivery, CTAB-LN dispersions were exposed to Human retinoblastoma cell line Y-79. The toxicity testing of the CTAB-LN dispersions was a fundamental tool to find the best CTAB concentration for development of these cationic LN, which was found to be 0.5wt% of CTAB.
Peer review: yes
URI: http://comum.rcaap.pt/handle/123456789/5691
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