Browsing by Author "Dias, E"
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- Ghrelin and obestatin: Different role in fetal lung development?Publication . Nunes, S; Nogueira-Silva, C; Dias, E; Moura, RS; Correia-Pinto, JGhrelin and obestatin are two proteins that originate from post-translational processing of the preproghrelin peptide. Various authors claim an opposed role of ghrelin and obestatin in several systems. Preproghrelin mRNA is significantly expressed in airway epithelium throughout lung development, predominantly during the earliest stages. The aim of this study was to evaluate the role of ghrelin and obestatin in fetal lung development in vitro. Immunohistochemistry studies were performed at different gestational ages in order to clarify the expression pattern of ghrelin, GHS-R1a, obestatin and GPR39 during fetal lung development. Fetal rat lung explants were harvested at 13.5 days post-conception (dpc) and cultured during 4 days with increasing doses of total ghrelin, acylated ghrelin, desacyl-ghrelin, ghrelin antagonist (D-Lys(3)-GHRP-6) or obestatin. Immunohistochemistry studies demonstrated that ghrelin, GHS-R1a, obestatin and GPR39 proteins were expressed in primitive rat lung epithelium throughout all studied gestational ages. Total and acylated ghrelin supplementation significantly increased the total number of peripheral airway buds, whereas desacyl-ghrelin induced no effect. Moreover, GHS-R1a antagonist significantly decreased lung branching. Finally, obestatin supplementation induced no significant effect in the measured parameters. The present study showed that ghrelin has a positive effect in fetal lung development through its GHS-R1a receptor, whereas obestatin has no effect on lung branching.
- Tratamento do Carcinoma da Próstata Metastizado: Surgimento de Novos Horizontes TerapêuticosPublication . Mota, P; Morais, N; Pimentel-Torres, J; Cordeiro, A; Dias, E; Cerqueira-Alves, M; Lima, EThe evolution in the treatment of metastatic prostate cancer currently provides to the urologist a wide range of therapeutic possibilities that have shown an improvement on overall survival and quality of life of patients. It is presented a clinical case in this article which reflects not only the effectiveness of these therapies but also their rapid evolution.
- Tratamento do Carcinoma da Próstata Metastizado: Surgimento de Novos Horizontes TerapêuticosPublication . Mota, P; Morais, N; Pimentel-Torres, J; Cordeiro, A; Dias, E; Cerqueira-Alves, M; Lima, EA evolução no tratamento do carcinoma da próstata metastizado proporciona atualmente ao Urologista uma vasta gama de possibilidades terapêuticas que comprovadamente melhoram a sobrevida e a qualidade de vida dos doentes. É apresentado um caso clínico neste artigo onde é patente não só a eficácia destas terapêuticas mas também a sua rápida evolução.
- Ureteroscopy-assisted Percutaneous Kidney Access Made Easy: First Clinical Experience with a Novel Navigation System Using Electromagnetic Guidance (IDEAL Stage 1)Publication . Lima, E; Rodrigues, PL; Mota, P; Carvalho, N; Dias, E; Correia-Pinto, J; Autorino, R; Vilaça, JLBACKGROUND: Puncture of the renal collecting system represents a challenging step in percutaneous nephrolithotomy (PCNL). Limitations related to the use of standard fluoroscopic-based and ultrasound-based maneuvers have been recognized. OBJECTIVES: To describe the technique and early clinical outcomes of a novel navigation system for percutaneous kidney access. DESIGN, SETTING, AND PARTICIPANTS: This was a proof-of-concept study (IDEAL phase 1) conducted at a single academic center. Ten PCNL procedures were performed for patients with kidney stones. SURGICAL PROCEDURE: Flexible ureterorenoscopy was performed to determine the optimal renal calyx for access. An electromagnetic sensor was inserted through the working channel. Then the selected calyx was punctured with a needle with a sensor on the tip guided by real-time three-dimensional images observed on the monitor. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: The primary endpoints were the accuracy and clinical applicability of the system in clinical use. Secondary endpoints were the time to successful puncture, the number of attempts for successful puncture, and complications. RESULTS AND LIMITATIONS: Ten patients were enrolled in the study. The median age was 47.1 yr (30-63), median body mass index was 22.85kg/m2 (19-28.3), and median stone size was 2.13cm (1.5-2.5cm). All stones were in the renal pelvis. The Guy's stone score was 1 in nine cases and 2 in one case. All 10 punctures of the collecting system were successfully completed at the first attempt without X-ray exposure. The median time to successful puncture starting from insertion of the needle was 20 s (range 15-35). No complications occurred. CONCLUSIONS: We describe the first clinical application of a novel navigation system using real-time electromagnetic sensors for percutaneous kidney access. This new technology overcomes the intrinsic limitations of traditional methods of kidney access, allowing safe, precise, fast, and effective puncture of the renal collecting system. PATIENT SUMMARY: We describe a new technology allowing safe and easy puncture of the kidney without radiation exposure. This could significantly facilitate one of the most challenging steps in percutaneous removal of kidney stones.