Browsing by Author "Oliveira, C"
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- 1ª Anestesia para craniotomia em doente acordado no Hospital de BragaPublication . Oliveira, C; Gomes, C; Cerqueira, I; Pereira, MJ
- Afinal os antecedentes cirúrgicos são importantes: a propósito de um caso clínicoPublication . Oliveira, C; Dinis, C; Gomes, C; Pereira, M
- Aspiração pulmonar de conteúdo gástrico durante a extracção de balão intra-gástricoPublication . Oliveira, C; Caridade, M; Gomes, C; Gomes, CM; Domingos, R
- Capsaicin 8% patch: the challengePublication . Oliveira, C; Gomes, C; Rebelo, V; Barbosa, M
- Capsaícina 8% - O novo desafio na dor crónicaPublication . Oliveira, C; Gomes, C; Rebelo, V; Barbosa, M
- Capsaícina 8%: o novo desafio na dor crónica: comunicação oralPublication . Gomes, C; Rebelo, V; Barbosa, M; Oliveira, C
- A case of Horner´s syndrome after internal jugular venous catheterization in a childPublication . Oliveira, C; Gomes, C; Farinha, F; Barros, F
- Characterization and genome sequencing of a Citrobacter freundii phage CfP1 harboring a lysin active against multidrug-resistant isolatesPublication . Oliveira, H; Pinto, G; Oliveira, A; Oliveira, C; Faustino, MA; Briers, Y; Domingues, L; Azeredo, JCitrobacter spp., although frequently ignored, is emerging as an important nosocomial bacterium able to cause various superficial and systemic life-threatening infections. Considered to be hard-to-treat bacterium due to its pattern of high antibiotic resistance, it is important to develop effective measures for early and efficient therapy. In this study, the first myovirus (vB_CfrM_CfP1) lytic for Citrobacter freundii was microbiologically and genomically characterized. Its morphology, activity spectrum, burst size, and biophysical stability spectrum were determined. CfP1 specifically infects C. freundii, has broad host range (>85 %; 21 strains tested), a burst size of 45 PFU/cell, and is very stable under different temperatures (-20 to 50 °C) and pH (3 to 11) values. CfP1 demonstrated to be highly virulent against multidrug-resistant clinical isolates up to 12 antibiotics, including penicillins, cephalosporins, carbapenems, and fluroquinoles. Genomically, CfP1 has a dsDNA molecule with 180,219 bp with average GC content of 43.1 % and codes for 273 CDSs. The genome architecture is organized into function-specific gene clusters typical for tailed phages, sharing 46 to 94 % nucleotide identity to other Citrobacter phages. The lysin gene encoding a predicted D-Ala-D-Ala carboxypeptidase was also cloned and expressed in Escherichia coli and its activity evaluated in terms of pH, ionic strength, and temperature. The lysine optimum activity was reached at 20 mM HEPES, pH 7 at 37 °C, and was able to significantly reduce all C. freundii (>2 logs) as well as Citrobacter koseri (>4 logs) strains tested. Interestingly, the antimicrobial activity of this enzyme was performed without the need of pretreatment with outer membrane-destabilizing agents. These results indicate that CfP1 lysin is a good candidate to control problematic Citrobacter infections, for which current antibiotics are no longer effective.
- Collecting system percutaneous access using real-time tracking sensors: first pig model in vivo experiencePublication . Rodrigues, PL; Vilaça, JL; Oliveira, C; Cicione, A; Rassweiler, J; Fonseca, J; Rodrigues, NF; Correia-Pinto, J; Lima, EPURPOSE: Precise needle puncture of the renal collecting system is a challenging and essential step for successful percutaneous nephrolithotomy (PCNL). This works aims to evaluate the efficiency of a new real-time electromagnetic tracking (EMT) system for in vivo kidney puncture. MATERIALS AND METHODS: Six anesthetized female pigs underwent ureterorenoscopies in order to place a catheter with an EMT sensor into the desired puncture site and to ascertain the success of puncture. Subsequently, a tracked needle with a similar EMT sensor was navigated into the sensor inside the catheter. Four punctures were performed by two surgeons in each pig: one in the kidney and one in the middle ureter, on both right and left pig sides. Number of attempts and time needed to evaluate the virtual trajectory and to perform the percutaneous puncture were outcomes measurements. RESULTS: Overall 24 punctures were easily performed without any complications. Surgeons required more time to evaluate the trajectory during ureteral puncture than kidney (median 15 versus 13 seconds, range 14 to 18 and 11 to 16 seconds, respectively; p= 0.1). The median renal and ureteral puncture time were 19 and 51 seconds respectively (range 14 to 45 and 45 to 67; p=0.003). Two attempts were needed to achieve a successful ureteral puncture. The presented technique demands presence of renal stone for testing. CONCLUSIONS: The proposed EMT solution for renal collecting system puncture proved to be highly accurate, simple and quicker. This method might represent a paradigm shift in percutaneous kidney access techniques.
- Glicogenose tipo Ia: avaliação e preparação pré-anestésica: caso clínicoPublication . Oliveira, C; Gomes, C; Santos, P; Fragoso, P; Crisóstomo, M; Antunes, H
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