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- Leukemia inhibitory factor in rat fetal lung development: expression and functional studiesPublication . Nogueira-Silva, C; Piairo, P; Carvalho-Dias, E; Peixoto, FO; Moura, RS; Correia-Pinto, JBACKGROUND: Leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) are members of the family of the glycoprotein 130 (gp130)-type cytokines. These cytokines share gp130 as a common signal transducer, which explains why they show some functional redundancy. Recently, it was demonstrated that IL-6 promotes fetal lung branching. Additionally, LIF has been implicated in developmental processes of some branching organs. Thus, in this study LIF expression pattern and its effects on fetal rat lung morphogenesis were assessed. METHODOLOGY/PRINCIPAL FINDINGS: LIF and its subunit receptor LIFRα expression levels were evaluated by immunohistochemistry and western blot in fetal rat lungs of different gestational ages, ranging from 13.5 to 21.5 days post-conception. Throughout all gestational ages studied, LIF was constitutively expressed in pulmonary epithelium, whereas LIFRα was first mainly expressed in the mesenchyme, but after pseudoglandular stage it was also observed in epithelial cells. These results point to a LIF epithelium-mesenchyme cross-talk, which is known to be important for lung branching process. Regarding functional studies, fetal lung explants were cultured with increasing doses of LIF or LIF neutralizing antibodies during 4 days. MAPK, AKT, and STAT3 phosphorylation in the treated lung explants was analyzed. LIF supplementation significantly inhibited lung growth in spite of an increase in p44/42 phosphorylation. On the other hand, LIF inhibition significantly stimulated lung growth via p38 and Akt pathways. CONCLUSIONS/SIGNIFICANCE: The present study describes that LIF and its subunit receptor LIFRα are constitutively expressed during fetal lung development and that they have an inhibitory physiological role on fetal lung
- Síndrome PFAPA - Caso clínicoPublication . Moreira, F; Pereira, G; Marçal, N; Guimarães, J; Miranda, DIntrodução - A Síndrome PFAPA (periodic fever, aphtous stomatitis, pharyngitis, cervical adenitis) caracteriza-se por episódios recorrentes de febre elevada, estomatite aftosa, adenite cervical e faringite que ocorre maioritariamente em crianças com idade inferior a cinco anos. A etiopatogenia é desconhecida e o diagnóstico é clínico e de exclusão. Com este trabalho pretendemos realizar uma revisão de literatura - clínica, diagnóstico e tratamento - complementada com a apresentação de um caso clínico. Materiais e métodos – Pesquisa bibliográfica e análise documental realizada através da base de dados da Medline, Pubmed e Ovid. Resultados - O uso de antibióticos ou cimetidina é ineficaz, enquanto a corticoterapia e a amigdalectomia, com ou sem adenoidectomia, reduzem a sintomatologia. Conclusões - A síndrome PFAPA é uma patologia rara, cujo reconhecimento pode ser difícil. O tratamento médico mais eficaz é a corticoterapia que, porém, não previne futuras recorrências. A terapêutica mais eficaz na resolução a longo prazo da sintomatologia é a amigdalectomia.
- Alimentação e nutrição do lactentePublication . Guerra, A; Rêgo, C; Silva, D; Ferreira, GC; Mansilha, H; Antunes, H; Ferreira, R
- Tuberculose em idade pediátrica: características, incidência e distribuição geográfica (2000-2010)Publication . Gonçalves, JP; Cerqueira, A; Machado, C; Carvalho, F; Cruz, S; Gonçalves, A; Martins, S
- Diversity in anti-N-methyl-D-aspartate receptor encephalitis: case-based evidencePublication . Pinho, J; Rocha, J; Rodrigues, M; Pereira, J; Maré, R; Ferreira, C; Lourenço, E; Beleza, PAntibodies against N-methyl-D-aspartate receptor (NMDAR) are identified in the form of immune-mediated encephalitis in which typical manifestations include neuropsychiatric symptoms, seizures, abnormal movements, dysautonomia and hypoventilation. The authors report two cases of anti-NMDAR encephalitis with different presentations and patterns of progression. The first patient presented with status epilepticus and later developed psychosis, pyramidal signs and diffuse encephalopathy. The second patient presented with acute psychosis followed a week later by seizures, dystonia, rigidity, oromandibular dyskinesias and dysautonomia. Possible mechanisms responsible for the clinical manifestations of this disease are discussed in light of recently described additional clinical and laboratory findings.
- Metacarpal solitary osteochondroma—case report of a rare conditionPublication . Duarte, RM; Ferreira, NV; Silva, LM; Rodrigues, LF; Ribeiro, JM; Vieira da Silva, M
- Impacto prognóstico da insuficiência mitral isquémica no EAM sem supraSTPublication . Ramos, V; Vieira, C; Galvão-Braga, C; Martins, J; Ribeiro, S; Rocha, S; Gaspar, A; Marques, J; Azevedo, P; Álvares-Pereira, M; Correia, A
- Diabetes e hiperglicemia: factores de prognóstico na pneumonia adquirida na comunidade – estudo retrospectivo em doentes admitidos no Hospital de BragaPublication . Fernandes, V; Ramalho, J; Santos, MJ; Oliveira, N; Pereira, M
- Capsaicin 8% patch: the challengePublication . Oliveira, C; Gomes, C; Rebelo, V; Barbosa, M
- Capsaícina 8% - O novo desafio na dor crónicaPublication . Oliveira, C; Gomes, C; Rebelo, V; Barbosa, M