Repository logo

Browsing by Author "Oliveira, R"

Now showing 1 - 2 of 2
  • Candida clinical species identification: molecular and biochemical methods
    Publication . Costa, AR; Silva, F; Henriques, M; Azeredo, J; Oliveira, R; Faustino, A
    In the last decade, the number and diversity of nosocomial Candida infections has increased significantly, resulting in an emergent need for rapid and accurate methods for Candida identification. Therefore, the aim of this study was to evaluate the performance of three biochemical systems (Auxacolor, ID32C, and Vitek 2 YST) for the identification of Candida species, comparing them with molecular identification (polymerase chain reaction and gel agarose electrophoresis). These methods were used to assess Candida spp. (229 clinical isolates) prevalence and distribution among clinical specimens. The biochemical methods with higher percentages of correct identification were Vitek 2 YST (79.6%) and Auxacolor (78.6%). However, overall the biochemical methods assayed differed from the molecular identification. Thus, due to their rapid and precise identification, molecular methods are promising techniques for Candida species identification in clinical laboratories. Candida albicans and Non Candida albicans Candida species had a similar prevalence (50.4 and 49.6%, respectively), corroborating the epidemiological shift observed for these pathogens in the recent years.
    2010Journal article Open access Show more
  • Late-onset Lennox-Gastaut syndrome as a phenotype of 15q11.1q13.3 duplication
    Publication . Rocha, J; Guerra, C; Oliveira, R; Dória, S; Rego, R; Rosas, MJ
    The clinical symptoms associated with chromosome 15q duplication syndrome manifest through a heterogeneous group of symptoms characterised by hypotonia, delay in motor skills and language development, cognitive and learning disabilities, autism spectrum disorder and refractory epilepsy. The late development of Lennox-Gastaut syndrome in patients with 15q11q13 duplication is a possibility that physicians should be aware of. We report the case of a 27-year-old man with a neurodevelopmental syndrome due to a 15q duplication, with intellectual disability, psychiatric disturbances, and an epileptic phenotype diagnosed as late-onset Lennox-Gastaut syndrome.
    2012Journal article Open access Show more